I’m currently trying to solve a mystery. The protein i work on is known to be necessary for how the bacteria can bind to the cells in our lungs. the first step to making us sick. But no one knows how this happens. I have a few theories (sometimes it helps to have come from a cancer background where cancer cells bidning is an important step of how they bind) so I am testing those out. I’ve waited patiently for my reagents/ingredients to arrive, so now it’s time to try out my theories.
My current experiment is looking at how the gene I’m interested in changes in levels when I add poly(I:C) – a virus like chemical. I want to see if the gene increases or decreases in response to this. The goal is to work out conditions where the gene is turned on and we see a lot of it and when it is turned off. Then I can start testing the on switch in those conditions.
At this very moment (21 Mar 2013, 11.33PM) I’m about to start performing a cell viability assay to measure how much cancer cells I managed to kill this week as a cumulative result of gene gun experiment. I work on cardiovascular diseases both at this stage I use several cancer cells as a model.
I’m currently trying to solve a mystery. The protein i work on is known to be necessary for how the bacteria can bind to the cells in our lungs. the first step to making us sick. But no one knows how this happens. I have a few theories (sometimes it helps to have come from a cancer background where cancer cells bidning is an important step of how they bind) so I am testing those out. I’ve waited patiently for my reagents/ingredients to arrive, so now it’s time to try out my theories.
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My current experiment is looking at how the gene I’m interested in changes in levels when I add poly(I:C) – a virus like chemical. I want to see if the gene increases or decreases in response to this. The goal is to work out conditions where the gene is turned on and we see a lot of it and when it is turned off. Then I can start testing the on switch in those conditions.
0
At this very moment (21 Mar 2013, 11.33PM) I’m about to start performing a cell viability assay to measure how much cancer cells I managed to kill this week as a cumulative result of gene gun experiment. I work on cardiovascular diseases both at this stage I use several cancer cells as a model.
0